Molecular biology lab manual lecture notes ebook free download pdf
Agarose gel electrophoresis is a widely used method that separates molecules based uponcharge, size and shape. It is particularly useful in sepa rating charged biomolecules such as DNA, RNA and proteins.Agarose gel electrophoresis possesses great resolving power, yet is relatively simple andstraightforward to perform. The gel is made by dissolving agarose powder in boilingbuffer solution. The solut ion is then cooled to approximately 55oC and poured into a mold containing a comb thatSamples are prepared for electrophoresis by mixing them withcomponents that will give the mixture density, such as glycerol or sucrose. This makes the samples denser than the electrophoresis buffer. These samples can then be loaded with a micropipette or transfer pipet into wells that were created in the gel by a template during casting. The dense samples sink through the buffer and remain in the wells.